|
|
Microfluidic models of cell membranes
Haluzová, Kateřina ; Dzik, Petr (referee) ; Pekař, Miloslav (advisor)
This work deals with artificial cellular membrane models, their various types, production and usage. It also summarises basic matherials for production of microfluidic chips. Experimental part is focused on optimalization of production of polydimethylsiloxane chip suitable for free-standing lipid bilayers. A preparation workflow was developed which includes casting on a silicon wafer, removing bubbles from the PDMS, curing it at 80 °C for 1 h, plasma bonding, annealing at 150 °C for 30 min and storage. Using fluorescence microscopy, the prepared chips were found to be strong and sealed.
|
|
|
Advanced membrane systems
Gjevik, Alžběta ; Márová, Ivana (referee) ; Mravec, Filip (advisor)
The diploma thesis deals with cellular membrane model preparation on microfluidic devices. It summarizes means of microfluidic device fabrication, phospholipid bilayer formation mechanisms, optimization techniques and characterization methods of those systems. It focuses on free-standing planar lipid bilayers which are easily accessible by a number of different characterization methods and at the same time exhibit good stability and variability. The aim of this work is to design and prepare a microfluidic chip on which a planar lipid bilayer can be prepared. It therefore presents microfluidic device prepared by soft lithography of PDMS adapted for model membrane formation by self-assembly of phospholipids at the interface of aqueous and organic phases created by the architecture of the microfluidic device. Formation of the model membrane was visualized by optical microscopy and fluorescence-lifetime imaging microscopy.
|
|
| |
|
|
Membrane modeling in microfludic chip
Konečná, Tereza ; Pekař, Miloslav (referee) ; Venerová, Tereza (advisor)
This work focuses on microfluidic chips suitable for modeling phospholipid membranes, their design, and possible applications. It also provides a more detailed specification of selected techniques for creating membranes on a chip. The experimental part deals with the production of chips from polydimethylsiloxane, the optimization of infusing of solutions into the chip, and the formation of membranes inside microfluidic channels using the selected model phospholipid – asolectin. The entire process of membrane modeling was visualized using optical and fluorescence microscopy. In conclusion, the success of the developed method is evaluated, including the suitability of asolectin for the formation of model membranes, and solutions to existing problems are proposed.
|
|
|
Microfludic chip development for phospholipid membrane modeling
Haluzová, Kateřina ; Pekař, Miloslav (referee) ; Venerová, Tereza (advisor)
The diploma thesis deals with the issue of phospholipid membranes and their modeling in a microfluidic environment. It describes the composition and its effect on the structure of cell membranes and defines a mathematical-physical model for characterizing the environment in microfluidic channels. Experimental part describes the production of a microfluidic chip and equipment required for reliable formation of membranes formed by L--phosphatidylcholine dissolved in hexadecane. The main emphasis is then placed on optimizing the conditions for the formation of this specific membrane model observed under a fluorescence microscope. We have succeeded in proving the correctness of the mathematical model and in partially describing the conditions under which the membrane is formed despite a large number of complications described in more detail in the discussion of this thesis.
|
|
|
Microfluidic models of cell membranes
Haluzová, Kateřina ; Dzik, Petr (referee) ; Pekař, Miloslav (advisor)
This work deals with artificial cellular membrane models, their various types, production and usage. It also summarises basic matherials for production of microfluidic chips. Experimental part is focused on optimalization of production of polydimethylsiloxane chip suitable for free-standing lipid bilayers. A preparation workflow was developed which includes casting on a silicon wafer, removing bubbles from the PDMS, curing it at 80 °C for 1 h, plasma bonding, annealing at 150 °C for 30 min and storage. Using fluorescence microscopy, the prepared chips were found to be strong and sealed.
|
|
|
Advanced membrane systems
Gjevik, Alžběta ; Márová, Ivana (referee) ; Mravec, Filip (advisor)
The diploma thesis deals with cellular membrane model preparation on microfluidic devices. It summarizes means of microfluidic device fabrication, phospholipid bilayer formation mechanisms, optimization techniques and characterization methods of those systems. It focuses on free-standing planar lipid bilayers which are easily accessible by a number of different characterization methods and at the same time exhibit good stability and variability. The aim of this work is to design and prepare a microfluidic chip on which a planar lipid bilayer can be prepared. It therefore presents microfluidic device prepared by soft lithography of PDMS adapted for model membrane formation by self-assembly of phospholipids at the interface of aqueous and organic phases created by the architecture of the microfluidic device. Formation of the model membrane was visualized by optical microscopy and fluorescence-lifetime imaging microscopy.
|
|
| |
guest ::
